期刊
DEVELOPMENTAL BIOLOGY
卷 293, 期 2, 页码 461-472出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2006.02.013
关键词
historic modification; germ cells; spermatogonia; epigenetics; stem cells; transcriptional repression
资金
- NICHD NIH HHS [U54 HD4254] Funding Source: Medline
- PHS HHS [5T32 H007453] Funding Source: Medline
Chromatin structure plays an important role in the regulation of gene expression. Methylation of lysine residues on historic tails is an epigenetic mark that influences chromatin repression when specifically imparted on lysines 9 and 27 of historic H3, and on lysine 20 of H4. Histone lysines can be mono-, di-, and trimethylated, and all three modification states have been identified in different nuclear domains. Correlation of these methylated historic states to different stages of cell differentiation, however, is not extensive. Mammalian spermatogenesis is a developmental process ideal for studying the epigenetic control of differentiation. Maintenance of spermatogonial stem cells requires the transcriptional repressor Plzf, but a role for historic methylation has not been established. Here we show that Plzf-expressing spermatogonia completely lack monomethyl-H3-K27 and monomethyl-H4-K20, and contain very little monomethyl-H3-K9. Dimethylated H3-K27 and H4-K20 are detected as punctate foci in Plzf-positive cells, but dimethyl-H3-K9 is absent. Trimethylated H3-K9 and H4-K20 exhibit a unique perinuclear distribution that coincides with Plzf expression, localizing to punctate foci in more differentiated spermatogonia. Loss of Plzf correlates with increased punctate distribution of trimethylated H3-K9 and H4-K20 at the expense of perinuclear localization. These data signify the possible importance of different historic lysine methylation states in the epigenetic control of spermatogenesis. (c) 2006 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据