期刊
EMBO JOURNAL
卷 25, 期 10, 页码 2230-2239出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.emboj.7601104
关键词
DNA translocation; helicase; motor protein; restriction enzyme; single molecule
资金
- Wellcome Trust [067439, 071432] Funding Source: Medline
Using a combination of single molecule and bulk solution measurements, we have examined the DNA translocation activity of a helicase, the Type I restriction modification enzyme EcoR124I. We find that EcoR124I can translocate past covalent interstrand crosslinks, inconsistent with an obligatory unwinding mechanism. Instead, translocation of the intact dsDNA occurs principally via contacts to the sugar-phosphate backbone and bases of the 30 - 50 strand; contacts to the 50 - 30 strand are not essential for motion but do play a key role in stabilising the motor on the DNA. A model for dsDNA translocation is presented that could be applicable to a wide range of other enzyme complexes that are also labelled as helicases but which do not have actual unwinding activity.
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