期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1116, 期 1-2, 页码 69-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.03.022
关键词
phenolic metabolites; LDL; olive oil; high-performance liquid chromatography; mass spectrometry; solid-phase extraction
A rapid method for detection and quantification of metabolites of specific olive oil phenolic compounds (hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate) in low-density lipoprotein (LDL) fractions by solid-phase extraction (SPE) and high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC/ESIMS/MS) is described. A 3 mu m particle size fast C-18 Luna column, 5 cm x 2.0 mm I.D., was used at a flow rate of 0.6 mL/min with a mobile phase consisting of 0.1% (v/v) formic acid (A) and acetonitrile (B). A linear gradient profile was used for separation at column temperature 40 degrees C. The proposed chromatographic procedure is rapid without loosing its separation efficiency and sensitivity. Validation proofs were carried out for the method described, showing a linear system (r > 0.99) and a recovery of 81.9 and 101.3% for hydroxytyrosol and homovanillic acid, respectively. The results show that this method is effective and can be used in routine analysis. (c) 2006 Elsevier B.V. All rights reserved.
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