期刊
JOURNAL OF LUMINESCENCE
卷 118, 期 2, 页码 238-244出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jlumin.2005.08.018
关键词
electrochemiluminescence; bioaffinity assay; immunoassay; hot electron; terbium; labels
类别
A homogeneous immunoassay of T4 was developed using a semiautomatic electrochemiluminometer modified from a commercially available fluorometer. In addition, from the same analyte panel an immunometric immunoassay of TSH at similar disposable oxide-covered aluminum rake electrodes was studied using this instrument both on homogeneous and heterogeneous basis. Detection was based on hot electron-induced cathodic electrochemiluminescence utilizing a commercially available Tb(III) chelate label. The assays were reasonably sensitive and comparison was made with other older methods. Thus, it is possible to develop both non-competitive and competitive immunoassays based on detection of hot electron-induced ECL of the labels. (c) 2005 Elsevier B.V. All rights reserved.
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