期刊
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
卷 11, 期 4, 页码 419-432出版社
SPRINGER
DOI: 10.1007/s00775-006-0088-7
关键词
coordination complexes of ferric ion; iron uptake; pyochelin; Pseudomonas aeruginosa; siderophores
Pyochelin, its analog 3''-nor-NH-pyochelin, and the related methyl hydroxamate, 2-(2'-hydroxyphenyl)-4,5-dihydrothiazol-4-carboxylic acid methoxymethyl amide, have been prepared together with their Fe(III) complexes. The solution stoichiometry and the coordination of the three Fe(III) complexes in methanol or buffered (pH similar to 2) 50:50 (v/v) methanol-water mixtures were determined using various spectroscopic methods: UV-vis absorption, X-ray absorption, extended X-ray absorption fine structure and electron paramagnetic resonance. All three systems showed both a 1:1 and 2:1 ligand-Fe(III) stoichiometry, but presented different coordination properties. Conditional formation constants (pH similar to 2) were determined for both the 1:1 and 2:1 complexes in all three systems. Computation of the coordination-conformational energies by semiempirical methods indicated that the coordination in the case of the 2:1 complexes of pyochelin-Fe(III) and 3''-nor-NH-pyochelin-Fe(III) was asymmetrical, with one molecule of pyochelin (or 3''-nor-NH-pyochelin) tetradentately coordinated (O1, N1, N2 and O3) to the Fe(III), and the second molecule bound bidentately (O1, N1 or N2, O3), to complete the octahedral geometry. In contrast, two molecules of the methyl hydroxamate each provided a set of tridentate ligand atoms in the formation of the 2:1 ligand-Fe(III) complex. These results are consistent with the role of pyochelin in the uptake of iron by the FptA receptor in the outer membrane of Pseudomonas aeruginosa and in several gram-negative bacteria.
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