期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 16, 期 11, 页码 2882-2885出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2006.03.012
关键词
activity-based probes; tryptase; chemical proteomics
A series of diphenylphosphonate-based probes were developed for the trypsin-like serine proteases. These probes selectively target serine proteases rather than general serine hydrolases that are targets for fluorophosphonate-based probes. This increased selectivity allows detection of low abundance serine proteases in complex proteomes using simple SIDS-PAGE methods. We present here the application of multiple probes in enzyme activity profiling of intact mast cells, a type of inflammatory cell implicated in allergy and autoimmune diseases. (c) 2006 Elsevier Ltd. All rights reserved.
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