期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 65, 期 3, 页码 628-631出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2005.09.016
关键词
blood cultures; selected ion flow tube-mass spectrometry; BacT/ALERT
We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms. (c) 2005 Elsevier B.V. All rights reserved.
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