Flectobacillus lacus sp nov., isolated from a highly eutrophic pond in Korea

A Gram-negative, almost straight, rod-shaped, non-motile bacterium, designated CL-GP79(T), was isolated from a highly eutrophic pond located within the campus of Seoul National University, Korea. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain CL-GP79(T) belongs to the family Flexibacteraceae, with Flectobacillus major as its closest relative (similarity of 95(.)7%). The cellular fatty acids consist mainly of C-16:1 omega 5c (mean SD, 26-9 +/- 10-8%), iso-C(15:0)2-OH and/or C-16:1 omega 7c (19(.)2 +/- 2(.)3%) and iSo-C-15:0 (12(.)1 +/- 1(.)3%). The DNA G + C content was found to be 38(.)3 mol%. Phenotypic, chemotaxonomic and phylogenetic analyses indicated that strain CL-GP79T could be assigned to the genus Flectobacillus, but could be distinguished from F major. The strain CL-GP79(T) therefore represents a novel species, for which the name Flectobacillus lacus sp. nov. is proposed, with CL-GP7 9T (=KCCM 4227(T)=JCM 13398(T)) as the type strain.