期刊
TISSUE ENGINEERING
卷 12, 期 6, 页码 1663-1673出版社
MARY ANN LIEBERT INC
DOI: 10.1089/ten.2006.12.1663
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资金
- NIDCR NIH HHS [R01 DE016523, R01 DE016523-01, DE016523] Funding Source: Medline
Hydrogels were prepared by copolymerizing a degradable macromer, poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) endcapped with methacrylate groups (PEG-LA-DM), with a nondegradable macromer, poly( ethylene glycol) dimethacrylate (PEGDM). Copolymer networks consisted of 100: 0, 83: 17, 67: 33, and 50: 50 PEGDM: PEG-LA-DM mass%, essentially creating scaffolds that exhibit 0, 17, 33, and 50% degradation over the time course of the experiment. Osteoblasts were photoencapsulated in these copolymer hydrogels and cultured for 3 weeks in vitro. Metabolic activity, proliferation, and alkaline phosphatase production were enhanced by an increase PEG-LA-DM content and corresponding degradation. Gene expression of the cultured osteoblasts, normalized to beta-actin, was analyzed, and osteopontin and collagen type I gene expression increased with degradation. Finally, as a measure of mineralized tissue formation, calcium and phosphate deposition was analyzed biochemically and histologically. Mineralization increased with increasing concentration of PEG-LA-DM and biochemically resembled that of hydroxyapatite.
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