期刊
JOURNAL OF CELL SCIENCE
卷 119, 期 11, 页码 2354-2361出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.02957
关键词
kinesin; mitosis; microtubule; catastrophe factor; flux; CLASP
类别
资金
- Medical Research Council [G0501718] Funding Source: Medline
- MRC [G0501718] Funding Source: UKRI
- Medical Research Council [G0501718] Funding Source: researchfish
The metaphase-spindle steady- state length occurs as spindle microtubules 'flux', incorporating new subunits at their plus ends, while simultaneously losing subunits from their minus ends. Orbit/ Mast/ CLASP is required for tubulin subunit addition at kinetochores, and several kinesins regulate spindle morphology and/ or flux by serving as microtubule depolymerases. Here, we use RNA interference in S2 cells to examine the relationship between Orbit and the four predicted kinesin-type depolymerases encoded by the Drosophila genome (Klp10A, Klp59C, Klp59D and Klp67A). Single depletion of Orbit results in monopolar spindles, mitotic arrest and a subsequent increase in apoptotic cells. These phenotypes are rescued by co-depleting Klp10A but none of the other three depolymerases. Spindle bipolarity is restored by preventing the spindle collapse seen in cells that lack Orbit, leading to functional spindles that are similar to controls in shape and length. We conclude that Klp10A exclusively antagonises Orbit in the regulation of bipolar spindle formation and maintenance.
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