In vitro antimicrobial activity of ozonized theobroma oil against Candida albicans

In this paper, a study of antimicrobial activity of ozonized theobroma oil against Candida albicans was carried out. Minimum inhibitory concentration and minimum fungicide concentration from ozonized theobroma oil for several peroxides indexes were determined. Mortality kinetics for peroxide index of 1200 mmol-eq/kg was carried out. Effect of contact time, peroxide index and ozonized theobroma oil concentration on Candida albicans mortality was studied. Under the conditions studied, minimum inhibitory concentration and minimum fungicide concentration values between 5 and 3.75 mg/mL and 11.58 and 5.78 mg/mL were obtained for peroxides indexes of 1002 and 1200 mmol-eq/kg of sample, respectively. Microorganism inactivation follows first order kinetics. An optimal concentration value of 93.75 mg/mL was obtained. Further increases of ozonized theobroma oil concentration did not exert any influence on mortality. Under the variables ranges studied all parameters have a significant effect on the mortality of Candida albicans, contact time being the most important factor.