期刊
ANNALS OF ONCOLOGY
卷 23, 期 3, 页码 638-646出版社
OXFORD UNIV PRESS
DOI: 10.1093/annonc/mdr290
关键词
cervical cancer; pelvic lymph node metastasis; EMT; prognosis; Sam68
类别
资金
- Foundation of Ministry of Science and Technology of China [81071780]
Background: This study was aimed at investigating the role and molecular mechanism of Sam68 in cervical cancer lymph node metastasis. Materials and methods: Sam68 expression profile was detected by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Short hairpin RNA interfering approach was employed to suppress endogenous Sam68 expression in cervical cancer cells to determine its role in metastasis and the possible mechanism. Results: Sam68 expression in cervical cancer was significantly up-regulated at both messenger RNA and protein levels compared with that in normal cervical tissues. The high expression level of Sam68 and its cytoplasmic localization were significantly associated with risk factors including pelvic lymph node metastasis (P < 0.001), and served as independent prognostic factors for predicting shortening of the overall survival time and disease-free survival time in patients with early-stage cervical cancer. Moreover, down-regulation of Sam68 in cervical cancer cells remarkably inhibited cellular motility and invasion. In addition, down-regulation of Sam68 reversed epithelial-mesenchymal transition through inhibiting the Akt/ GSK-3 beta/Snail pathway. Conclusion: This study demonstrated that Sam68 could induce cervical cancer lymph node metastasis through regulating epithelial-mesenchymal transition, and Sam68 expression profile possessed the potential to serve as predictors of pelvic lymph node metastasis in cervical cancer patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据