期刊
PLOS BIOLOGY
卷 4, 期 6, 页码 1069-1078出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.0040185
关键词
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资金
- NIAID NIH HHS [R01 AI055977-03, R01 AI044239-07, R01 AI055977, R37 AI033493, R01 AI055977-02, R01 AI044239-06A2, R37 AI033493-12, R37 AI033493-11, R01 AI044239] Funding Source: Medline
We show using several methodologies that the Gram-negative, diplococcal-bacterium Neisseria gonorrhoeae has more than one complete genome copy per cell. Gene dosage measurements demonstrated that only a single replication initiation event per chromosome occurs per round of cell division, and that there is a single origin of replication. The region containing the origin does not encode any genes previously associated with bacterial origins of replication. Quantitative PCR results showed that there are on average three genome copies per coccal cell unit. These findings allow a model for gonococcal DNA replication and cell division to be proposed, in which a minimum of two chromosomal copies exist per coccal unit within a monococcal or diplococcal cell, and these chromosomes replicate in unison to produce four chromosomal copies during cell division. Immune evasion via antigenic variation is an important mechanism that allows these organisms to continually infect a high risk population of people. We propose that polyploidy may be necessary for the high frequency gene conversion system that mediates pilin antigenic variation and the propagation of N. gonorrhoeae within its human hosts.
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