Site-specific scission of lambda phage genomic DNA by Ce(IV)/EDTA-based artificial restriction DNA cutter

Lambda phage genomic DNA (48502 base pairs) was site-selectively hydrolyzed by artificial restriction DNA cutter (ARCUT), which we have developed by combining Ce(IV)/EDTA and pseudo-complementary PNA. PNA satisfactorily invaded lambda DNA and activated the target site for the scission by Ce(IV)/EDTA.