期刊
VIROLOGY
卷 349, 期 2, 页码 245-253出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2006.03.007
关键词
West Nile virus; Flavivirus; NS4B protein; attenuated phenotype; cysteine
类别
资金
- NIAID NIH HHS [T32 AI 7526] Funding Source: Medline
West Nile virus (WNV) NS4B is a small hydrophobic nonstructural protein that is hypothesized to participate both in viral replication and evasion of host innate immune defenses. The protein has four cysteine residues (residues 102, 120, 227, and 237). Since cysteines are often critical for the function of proteins, each of the four cysteine residues found in WNV NS4B was mutated to serine by site-directed mutagenesis. While three of these substitutions had little effect on replication or mouse virulence phenotypes, the C102S mutation was associated with a temperature-sensitive phenotype at 41 degrees C as well as attenuation of the neuroinvasive and neurovirulence phenotypes in mice. (c) 2006 Elsevier Inc. All rights reserved.
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