期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1117, 期 2, 页码 163-169出版社
ELSEVIER
DOI: 10.1016/j.chroma.2006.03.084
关键词
molecularly imprinted polymer; monolithic column; tetrahydropalmatine; chiral separation; on-line; solid-phase extraction; Corydalis yanhusuo
A method for direct determination of DL-tetrahydropalmatine (DL-THP) in Corydalis yanhusuo, a traditional Chinese herb, by L-THP imprinted monolithic precolumn on-line/off-line coupling with reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The L-THP imprinted monolithic column has been prepared by in situ polymerization using methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) as functional monomer and cross-linker, respectively. With the optimization of chromatographic conditions, such as mobile phase composition, flow rate, column temperature and sample loading, for the separation of enantiomer, DL-THP was base-line separated on the MIP. The imprinted monolithic column was used as a precolumn for fractionation of the C. yanhusuo extract. Both the non-retained and retained fractions were separated by RP-HPLC. Meanwhile, the D-THP and L-THP can be detected in the non-retained and retained fractions, respectively. Additionally, direct determination of L-THP using molecularly imprinted monolith on-line coupling with a reversed-phase column was acquired. (c) 2006 Elsevier B.V. All rights reserved.
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