期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 40, 期 12, 页码 2112-2125出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2006.02.009
关键词
nuclear factor-kappa B; reactive nitrogen species; nitric oxide; peroxynitrite; skeletal muscle cells; protein degradation; MMPs; ubiquitin-proteasome
Recently, a role for NF-kappa B in upregulation of proteolytic systems and protein degradation has emerged. Reactive nitrogen species (RNS) have been demonstrated to induce NF-kappa B activation. The aim of this study was to investigate whether RNS caused increased proteolysis in skeletal muscle cells, and whether this process was mediated through the activation of NF-kappa B. Fully differentiated L6 myotubes were treated with NO donor SNAP, peroxynitrite donor SIN-1, and authentic peroxynitrite, in a time-dependent manner. NF-kappa B activation, the activation of the ubiquitin-proteasome pathway and matrix metalloprotemases, and the levels of muscle-specific proteins (myosin heavy chain and telethonin) were investigated under the conditions of nitrosative stress. RNS donors caused NF-kappa B activation and increased activation of proteolytic systems, as well as the degradation of muscle-specific proteins. Antioxidant treatment, tyrosine nitration inhibition, and NF-kappa B molecular inhibition were proven effective in downregulation of NF-kappa B activation and slowing down the degradation of muscle-specific proteins. Peroxynitrite, but not NO, causes proteolytic system activation and the degradation of muscle-specific proteins in cultured myotubes, mediated through NF-kappa B. NF-kappa B inhibition by antioxidants, tyrosine nitration, and molecular inhibitors may be beneficial for decreasing the extent of muscle damage induced by RNS. (c) 2006 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据