期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 253, 期 1-2, 页码 58-64出版社
ELSEVIER
DOI: 10.1016/j.ijms.2006.02.015
关键词
proton-transfer reaction mass spectrometry; volatile organic compound; lipid peroxidation; aldehydes; in vivo; human skin
Proton-transfer reaction mass spectrometry (PTR-MS) was used to study ultraviolet (UV) light-induced lipid peroxidation in human skin, in vivo. Emissions of volatile organic compounds (VOCs) in the mass range between 20 and 150 amu in the headspace of the skin of 16 healthy volunteers were monitored before, during and after irradiation in an on-line and non-invasive fashion. From these experiments, five volatile substances were found to reflect the damage caused by UV-radiation. The two major compounds (monitored at mass 45 and 59 amu) we re identified as acetaldehyde and propanal using a combination of Tenax-based gas chromatographic pre-separation with PTR-MS. The other volatiles (with characteristic ions at, among others, masses 73 and 87 amu) could not be identified. Simultaneous measurement of the established lipid peroxidation biomarker ethene using laser-based photoacoustic trace gas detection revealed a similar pattern and statistically significant correlations between VOC production measured with PTR-MS and ethene. Variations in UV-radiation intensity were reflected by the amount of acetaldehyde and propanal emitted from the skin. Our results show that acetaldehyde and propanal can be used as biomarkers, for lipid peroxidation. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据