期刊
CURRENT BIOLOGY
卷 16, 期 12, 页码 1211-1216出版社
CELL PRESS
DOI: 10.1016/j.cub.2006.04.034
关键词
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资金
- NEI NIH HHS [R01 EY012837-03, R01 EY012837-02, EY12837, R01 EY012837] Funding Source: Medline
- NIDDK NIH HHS [DK069605, R01 DK084725] Funding Source: Medline
- NIGMS NIH HHS [R01 GM070862-01A1, GM07767, R01 GM070862] Funding Source: Medline
Nonmotile cilia on olfactory sensory neurons (OSNs) compartmentalize signaling molecules, including odorant receptors and cyclic nucleotide-gated (CNG) channels, allowing for efficient, spatially confined responses to sensory stimuli [1-3]. Little is known about the mechanisms of the ciliary targeting of olfactory CNG channels, composed of three subunits: CNGA2, CNGA4, and CNGB1b [4]. Recent reports suggest that subunit composition of the retinal CNG channel influences localization, leading to disease [5, 6]. However, the mechanistic role of subunits in properly targeting native olfactory CNG channels remains unclear. Here, we show that heteromeric assembly with CNGB1b, containing a critical carboxy-terminal motif (RVxP), is required for ciliary trafficking of olfactory CNG channels. Movement of proteins within the cilia is governed by intraflagellar transport (IFT), a process that facilitates bidirectional movement of cargo along microtubules [7,8]. Work in C. elegans has established that heterotrimeric and homodimeric kinesin-2 family members play a critical role in anterograde transport [9-11]. In mammalian systems, the heterotrimeric KIF3a/KIF3b/KAP-3 complex plays a clear role in IFT; however, no role has been established for KIF17, the mammalian homolog of OSM-3 [12]. Here, we demonstrate that KIF17 is required for olfactory CNG channel targeting, providing novel insights into mechanisms of mammalian ciliary transport.
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