期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 359, 期 5, 页码 1182-1195出版社
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2006.04.063
关键词
RNA polymerase; sigma factors; DNA opening; T7 gene protein (gp2); promoter stability
资金
- NIGMS NIH HHS [GM 64530] Funding Source: Medline
The bacterial RNA polymerase (RNAP) is a multi-subunit, structurally flexible, complex molecular machine, in which activities associated with DNA opening for transcription-competent open promoter complex (OC) formation reside in the catalytic beta and beta' subunits and the dissociable sigma subunit. OC formation is a multi-step process that involves several structurally conserved mobile modules of beta, beta', and sigma. Here, we present evidence that two flexible modules of beta', the beta' jaw and the beta' clamp and a conserved regulatory Region I domain of sigma(54), jointly contribute to the maintenance of stable DNA strand separation around the trancription start site in OCs formed at sigma(54)-dependent promoters. Clearly, regulated interplay between the mobile modules of the beta' and the sigma subunits of the RNAP appears to be necessary for stable OC formation. (c) 2006 Elsevier Ltd. All rights reserved.
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