期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 26, 页码 9940-9945出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0509185103
关键词
proteomics; transcription factor; yeast
资金
- NCI NIH HHS [R01 CA077808, CA 77808] Funding Source: Medline
- NIGMS NIH HHS [GM 063803, R01 GM063803, R01 GM078222] Funding Source: Medline
Analyses of whole-genome sequences and experimental data sets have revealed a large number of DNA sequence motifs that are conserved in many species and may be functional. However, methods of sufficient scale to explore the roles of these elements are lacking. We describe the use of protein arrays to identify proteins that bind to DNA sequences of interest. A microarray of 282 known and potential yeast transcription factors was produced and probed with oligonucleotides of evolutionarily conserved sequences that are potentially functional. Transcription factors that bound to specific DNA sequences were identified. One previously uncharacterized DNA-binding protein, YjI103, was characterized in detail. We defined the binding site for this protein and identified a number of its target genes, many of which are involved in stress response and oxidative phosphorylation. Protein microarrays offer a high-throughput method for determining DNA-protein interactions.
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