期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 345, 期 2, 页码 573-580出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.04.096
关键词
NAADP(-); 2 '-phospho-cyclic ADP-ribose; nicotinic acid; ADP-ribosyl cyclase; NADP(+); HL-60 cells
ADP-ribosyl cyclases (ADPRCs) are present from lower Metazoa to mammals and synthesize the Ca2+-active (di)nucleotides cyclic ADP-ribose (cADPR), NAADP(+), and ADP-ribose (ADPR), involved in the regulation of important cellular functions. NAADP(+) can be synthesized by ADPRCs from NADP(+) through a base-exchange reaction, which substitutes nicotinamide for nicotinic acid (NA). Here we demonstrate that ADPRCs from both lower and higher Metazoa (including human CD38) can also synthesize NAADP(+) starting from 2'-phospho-cyclic ADP-ribose (cADPRP) and NA. Comparison, on the two substrates cADPRP and NADP(+), of the relative rates of the reactions introducing NA and hydrolyzing/cyclizing the substrate, respectively, indicates that with all ADPRCs tested cADPRP is preferentially transformed into NAADP, while NADP(+) is preferentially cyclized or hydrolyzed to cADPRP/2'-pliosplio-ADP-ribose. cADPRP was detectable in retinoic acid-differentiated, CD38(+) HL-60 cells, but not in Undifferentiated, CD38(-) cells. These results suggest that cADPRP may be a NAADP(+) precursor in ADPRC(+) cells. (c) 2006 Elsevier Inc. All rights reserved.
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