期刊
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
卷 126, 期 -, 页码 380-389出版社
ELSEVIER
DOI: 10.1016/j.autneu.2006.02.030
关键词
inflammation; chemokines; urothelium; western blot; ELISA
资金
- NIDDK NIH HHS [R29 DK051369, DK065989, R56 DK060481, R01 DK060481, R01 DK065989, R01 DK051369, DK051369, DK060481] Funding Source: Medline
- NINDS NIH HHS [R01 NS040796, NS040796] Funding Source: Medline
Alterations in the expression of the chemokine, fractalkine (CX3CL1), were examined in the urinary bladder after cyclophosphamide (CYP)-induced cystitis of varying duration: acute (4 h or 48 la), or chronic (10 day). CYP-induced cystitis significantly (p <= 0.01) increased fractalkine protein expression in the urinary bladder with acute (48 h) and chronic CYP treatment. Western blot analysis also demonstrated significantly (p <= 0.01) increased fractalkine expression in the whole urinary bladder with acute (1.5-2.2-fold) and chronic (3-fold) CYP-induced cystitis. Immunohistochemistry for fractalkine-immunoreactivity revealed little fractalkine-IR in control or acute (4 h) CYP-treated rat urinary bladders except in a vascular bed but showed no colocalization with nerve fibers in the suburothelial plexus in any experimental group. However, expression was significantly (p < 0.001) upregulated in the urothelium with 48 h or chronic CYP treatment. Similarly, fractalkine receptor (CX3CR1)-IR was significantly (p < 0.001) upregulated in the urothelium with 48 h or chronic CYP treatment. These studies demonstrated upregulation of the chemokine, fractalkine, in the urinary bladder and specifically in the urothelium with CYP-induced cystitis. Chemokines, and specifically, fractalkine, may be another class of neuromodulatory agents upregulated in the urinary bladder that can affect micturition function and sensory processing with cystitis and may represent novel, drug targets for cystitis. (c) 2006 Elsevier B.V. All rights reserved.
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