期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 144, 期 3, 页码 365-371出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2006.03.014
关键词
antioxidant enzyme; bumblebee; Bombus ignitus; cDNA cloning; Cu,Zn superoxide dismutase (SOD1); insect; gene structure; oxidative stress; paraquat; reactive oxygen species (ROS)
A Cu,Zn superoxide dismutase (SOD1) gene was cloned from the bumblebee, Bombus ignitus. The SOD1 gene of B. ignitus spans 1317 bp and consists of three introns and four exons encoding 151 amino acid residues. The B. ignitus SOD1 (BiSOD1) possesses the typical metal-binding ligands of six histidines and one aspartic acid common to SOD Is. The deduced amino acid sequence of the BiSOD1 cDNA showed 82% identity to Apis mellifera SODI and 68-64% to SODI sequences from other insects. Northern blot analysis revealed the presence of BiSOD1 transcripts in all tissues examined. When paraquat (methyl viologen), a free radical-inducing agent, was injected into the body cavity of B. ignitus workers, BiSOD1 mRNA expression was up-regulated in the fat body. In addition, the expression levels of BiSOD1 mRNA in the fat body significantly increased when B. ignitus workers were exposed to low (4 degrees C) or high (37 degrees C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that BiSOD1 possibly protects against oxidative stress caused by extreme temperatures and bacterial infection. (c) 2006 Elsevier Inc. All rights reserved.
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