期刊
CANCER BIOLOGY & THERAPY
卷 5, 期 7, 页码 842-847出版社
LANDES BIOSCIENCE
DOI: 10.4161/cbt.5.7.2840
关键词
siRNA; estrogen receptor alpha; apoptosis; breast cancer; cancer gene therapy
类别
Breast cancer, the most common malignancy in women, has a known association with the steroid hormone estrogen. Estrogen receptor alpha (ER alpha) plays an important role in the clinical care of breast cancer patients, both as a prognostic factor and as a therapeutic target. Here, we show that a small interfering RNA (siRNA) against ER alpha downregulates ER alpha expression in human MCF-7 and Bcap-37 breast cancer cells, causing a significant decrease in breast cancer cell proliferation. Tumor cells locking ER alpha expression grew at a much slower rate than did control cells in vitro. Moreover, ER alpha knockdown in breast cancer cells resulted in decreased, even completely abrogated tumor growth in BALB/c nude mice, providing direct evidence for an essential role of ER alpha in breast cancer growth. Our results suggest siRNA-mediated gene silencing of ER alpha may impair tumorigenicity, and even suppress the tumor growth.
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