期刊
LABORATORY ANIMALS
卷 40, 期 3, 页码 275-281出版社
ROYAL SOC MEDICINE PRESS LTD
DOI: 10.1258/002367706777611479
关键词
catheterization; infusion; metabolic phenotyping; mouse models; stable isotope
资金
- NIDDK NIH HHS [DK54450] Funding Source: Medline
We have developed and validated catheterization protocols in mice that allow for simultaneous infusion and sampling. A sampling catheter was inserted in the lateral vein of the tail, while the animals were infused either intravenously or intragastrically through a second catheter placed in the contralateral lateral vein or via an intragastric catheter, respectively. The applicability of these methods of infusion and blood sampling were validated by conducting urea kinetics utilizing stable isotopes. These non-surgical procedures are non-invasive, inexpensive, fast to perform and animals do not require a recovery period before their use.
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