期刊
APPLIED SPECTROSCOPY
卷 60, 期 7, 页码 752-757出版社
SAGE PUBLICATIONS INC
DOI: 10.1366/000370206777887035
关键词
laccase immobilization; surface-enhanced Raman scattering; SERS; resonance Raman
Resonance Raman (RR) and surface-enhanced Raman scattering (SERS) spectroscopy have been used to study immobilization of laccase on self-assembled monolayers (SAMs) of thiols containing carboxylic and amino groups, deposited on silver and gold electrodes. A new, indirect way of monitoring laccase bound to the thiol-coated Ag and An surfaces is presented. It was demonstrated that by recording the resonance Raman spectra of the colored product of the oxidation of syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehydeazine) by laccase in the presence of molecular oxygen, one may easily confirm binding as well as enzymatic activity of laccase immobilized on the SAMs modified silver and gold surfaces.
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