期刊
BIOLOGY OF THE CELL
卷 98, 期 7, 页码 415-425出版社
WILEY
DOI: 10.1042/BC20050097
关键词
basal body; electron microscopy; Paramecium; tomography
类别
Background information. Transmission electron tomography is becoming a powerful tool for studying subcellular components of cells. Classical approaches for electron tomography consist of recording images along a single-tilt axis. This approach is being improved by dual-axis reconstructions and/or high-tilt devices (tilt angle >+/- 60 degrees) on microscopes to compensate part of the information loss due to the 'missing wedge' phenomena. Results. In the present work we have evaluated the extension of the dual-axis technique to a multiple-axis approach, and we demonstrate a freely available plug-in for the Java-based freeware image-analysis software ImageJ. Our results from phantom and experimental data sets from Paramecium tetraurelia epon-embedded sections have shown that multiple-axis tomography achieves results equivalent to those obtained by dual-axis approach without the requirement for high-tilt devices. Conclusions. This new approach allows performance of high-resolution tomography, avoiding the need for high-tilt devices, and therefore will increase the access of electron tomography to a larger community.
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