期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 70, 期 7, 页码 1773-1775出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.50626
关键词
1,6-beta-D-glucanase; beta-1,6-glucanase gene; beta-1,6-glucanase disruptant; fungal beta-1,6-glucanase; fungal cell wall
The 1,6-beta-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.
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