期刊
ANNALS OF MICROBIOLOGY
卷 65, 期 3, 页码 1341-1350出版社
BMC
DOI: 10.1007/s13213-014-0972-2
关键词
Streptomyces; Taxonomy; Antistaphylococcal activities; Di-(2-ethylhexyl) phthalate
An actinomycete strain designated G60 was isolated from a Saharan soil sample in Ghardaia, Algeria, by a dilution agar plating method using chitin-vitamin agar medium supplemented with penicillin. Morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Analysis of the 16S rDNA sequence showed an identity level within Streptomyces species, with S. coerulescens ISP 5146(T) and S. bellus ISP 5185(T) the most closely related (100 % for each). However, the comparison of the morphological and physiological characteristics of the strain with those of the two nearest species showed significant differences. Strain G60 had a very strong activity against pathogenic staphylococci, including methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus (VRSA) S1. One antimicrobial compound was extracted by n-hexane from the ISP2 culture medium at 5 days of fermentation culture and purified by HPLC. The chemical structure of the compound was determined after spectroscopic (H-1 NMR, C-13 NMR, H-1-H-1 COSY and H-1-C-13 HMBC spectra), and spectrometric (mass spectrum) analyses. The bioactive compound was identified as di-(2-ethylhexyl) phthalate.
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