期刊
PLANT CELL REPORTS
卷 25, 期 7, 页码 636-642出版社
SPRINGER
DOI: 10.1007/s00299-006-0129-2
关键词
Cedrus; DNA methylation; in vitro storage; minimal growth; RAPD
We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.
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