期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 35, 页码 19530-19535出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b05232
关键词
RNAi; dsRNA; mosquito; SRC; SNF7; CQD; Chitosan
资金
- National Institutes of Health [GM070559-09]
- National Science Foundation (NSF)
- Environmental Protection Agency (EPA) under NSF [EF-0830093, DBI-1266252]
In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.
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