期刊
ANNALS OF MICROBIOLOGY
卷 62, 期 4, 页码 1757-1767出版社
BMC
DOI: 10.1007/s13213-012-0434-7
关键词
Used lubricating oil; Biodegradation; Surface tension; Biosurfactant; Polyaromatic hydrocarbon
资金
- TRF/BIOTEC Special Program for Biodiversity Research and Training grant [BRT R_150005]
- Graduate school, Prince of Songkla University
- Prince of Songkla University [AGR5111990022S]
Consortia of used lubricating oil-degrading bacteria were isolated from oil-contaminated soil. The most active consortia in the assimilation of used lubricating oil were SC-9. This consortia contained four bacterial isolates based on culture-dependent technique. They were identified as Agrobacterium tumefaciens, Bacillus cereus, Chryseobacterium sp., and Sphingobacterium multivorum based on 16S rDNA sequence analysis. The SC-9 consortia showed 40.5% oil-degrading activity within 7 days. The addition of molasses to the liquid medium had a positive effect and there was an increase in the percentage of oil degradation and a greater in reduction surface tension. The ability of selected bacteria to degrade oil increased in a comparable trend as its biosurfactant production increased. Oil-degrading bacterial isolates that display substantial potential for production of biosurfactants can be applied in the bioremediation of soils contaminated with petroleum hydrocarbons.
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