期刊
ANNALS OF MICROBIOLOGY
卷 62, 期 3, 页码 1089-1098出版社
SPRINGER
DOI: 10.1007/s13213-011-0350-2
关键词
Antifungal activity; Chitosanase; Cyanobacteria; HPLC; q-RT PCR
资金
- AMAAS Network project on Microorganisms
- Indian Council of Agricultural Research (ICAR), New Delhi
A novel antifungal chitosanase from Anabaena fertilissima, strain RPAN1, was characterized as a prelude to its use in biocontrol. The culture grown at 8:16 h L:D photoperiod showed highest chitosanase/antifungal activity under environmental and nutritional conditions of 43 mu M of P level, pH 9.0 and temperature of 27A degrees C. The transcriptional level of chitosanase encoding gene (cho) measured using quantitative real-time PCR (qRT-PCR) also indicated increased expression levels under the same optimized conditions. Under these conditions, cho encoding chitosanase was purified which exhibited a specific activity of 822 U/mg. The chitosanase activity measured using different substrates showed the highest activity against colloidal chitosan. HPLC profile of the products of enzyme activity with different chitosan oligosaccharides revealed the production of dimer units (GlcN)(2) or more, confirming the endo-type nature of the purified chitosanase. The optimum pH and temperature of the purified enzyme was 7.5 and 27A degrees C, respectively. Further, the enzyme was stable in the pH range of 5.5-9.0 up to 12 h and temperature between 27 and 50A degrees C up to 3 h. The enzyme was strongly inhibited by Ag+, Fe3+ and Hg2+ and stimulated by Cu+2 and Zn2+. The investigation revealed significant features regarding the stability of the chitosanase enzyme from A. fertilissima under a broad range of pH and temperature which can help in its effective use in biocontrol.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据