期刊
ANNALS OF MICROBIOLOGY
卷 61, 期 1, 页码 179-183出版社
SPRINGER
DOI: 10.1007/s13213-010-0089-1
关键词
Tyrosine decarboxylase; Agmatine deiminase; Lactobacillus brevis; Biogenic amine; Stress
资金
- EU commission [211441]
Lactobacillus brevis IOEB 9809, isolated from red wine, is able to produce both tyramine and putrescine. Using a real-time quantitative reverse transription PCR (qRT-PCR) we analyzed the relative expression of L. brevis IOEB 9809 tdc and aguA1 genes, encoding, respectively, a tyrosine decarboxylase and an agmatine deiminase enzyme. Relative gene expression was monitored either during the different growth phases under optimal conditions or under abiotic stress commonly found in wine. The tdc and aguA1 specific cDNA were amplified with specific primers. Our results indicate that tdc and aguA1 genes are differently expressed during the different growth phases and transiently induced by ethanol (12% v/v) and acidic stresses (pH 3.2 and 5.0).
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