期刊
ANNALS OF MICROBIOLOGY
卷 60, 期 1, 页码 59-64出版社
SPRINGER
DOI: 10.1007/s13213-009-0002-y
关键词
Bacillus cereus; Esterase; 1-chloro-3-(1-napthyloxy)-2-acetoxypropanol; Propranolol
资金
- State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences [SKLMR-080604]
An esterase from Bacillus cereus that hydrolyzes 1-chloro-3-(1-napthyloxy)-2-acetoxypropanol was purified to homogeneity. After purification, the molecular mass of the esterase was determined as 43 kDa by SDS-PAGE, and estimated as 45 kDa using gel filtration, suggesting that the enzyme is a monomer. The optimum pH and temperature for activity of the enzyme were 7.0 and 40 degrees C respectively. The N-terminal sequence was determined. Whole cells of this strain were applied to the resolution of 1-chloro-3-(1-napthyloxy)-2-acetoxypropanol.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据