期刊
FREE RADICAL RESEARCH
卷 40, 期 7, 页码 731-739出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760600632545
关键词
Alzheimer's disease; Parkinson's disease; electron spin resonance; reactive oxygen species; hydroxyl radical; hydrogen peroxide
资金
- Wellcome Trust [/GR065764AIA] Funding Source: Medline
There is clear evidence implicating oxidative stress in the pathology of many neurodegenerative diseases. Reactive oxygen species (ROS) are the primary mediators of oxidative stress, and hydrogen peroxide, a key ROS, is generated during aggregation of the amyloid proteins associated with some of these diseases. Hydrogen peroxide is catalytically converted to the aggressive hydroxyl radical in the presence of Fe(11) and Cu(I), which renders amyloidogenic proteins such as P-amyloid and alpha-synuclein (implicated in Alzheimer's disease (AD) and Parkinson's disease (PD), respectively) vulnerable to self-inflicted hydroxyl radical attack. Here, we report some of the peptide-derived radicals, detected by electron spin resonance spectroscopy employing sodium 3,5-dibromo-4-nitrosobenzenesulfonate as a spin-trap, following hydroxyl radical attack on A beta (1 - 40), alpha-synuclein and some other related peptides. Significantly, we found that sufficient hydrogen peroxide was self-generated during the early stages of aggregation of A beta(1-40) to produce detectable peptidyl radicals, on addition of Fe(11). Our results support the hypothesis that oxidative damage to AP (and surrounding molecules) in the brain in AD could be due, at least in part, to the self-generation of ROS. A similar mechanism could operate in PD and some other protein conformational disorders.
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