期刊
INFECTION AND IMMUNITY
卷 74, 期 7, 页码 3817-3824出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00317-06
关键词
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资金
- NIAID NIH HHS [K08 AI057999, R01 AI037532, R01 AI37532, T32 AI052070, R01 AI066087, R01 AI025780-22, R01 AI066087-02, 5K08 AI57999, R01 AI066087-01, R01 AI025780-20, R01 AI25780, R01 AI025780, R01 AI025780-21] Funding Source: Medline
Dendritic cells (DC) have been shown to phagocytose and kill Cryptococcus neoformans in vitro and are believed to be important for inducing protective immunity against this organism. Exposure to C. neoformans occurs mainly by inhalation, and in this study we examined the in vivo interactions of C. neoformans with DC in the lung. Fluorescently labeled live C. neoformans and heat-killed C. neoformans were administered intra-nasally to C57BL/6 mice. At specific times postinoculation, mice were sacrificed, and lungs were removed. Single-cell suspensions of lung cells were prepared, stained, and analyzed by microscopy and flow cytometry. Within 2 It postinoculation, fluorescently labeled C. neoformans had been internalized by DC, macrophages, and neutrophils in the mouse lung. Additionally, lung DC from mice infected for 7 days showed increased expression of the maturation markers CD80, CD86, and major histocompatibility complex class II. Finally, ex vivo incubation of lung DC from infected mice with Cryptococcus-specific T cells resulted in increased, interleukin-2 production compared to the production by DC from naive mice, suggesting that there was antigen-specific T-cell activation. This study demonstrated that DC in the lung are capable of phagocytosing Cryptococcus in vivo and presenting antigen to C. neoformans-specific T cells ex vivo, suggesting that these cells have roles in innate and adaptive pulmonary defenses against cryptococcosis.
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