期刊
JOURNAL OF CELL BIOLOGY
卷 174, 期 1, 页码 27-38出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200603083
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资金
- NCI NIH HHS [P30 CA034196, CA034196] Funding Source: Medline
- NICHD NIH HHS [R01 HD041066, HD41066] Funding Source: Medline
Specific mammalian genes functionally and dynamically associate together within the nucleus. Yet, how an array of many genes along the chromosome sequence can be spatially organized and folded together is unknown. We investigated the 3D structure of a well-annotated, highly conserved 4.3-Mb region on mouse chromosome 14 that contains four clusters of genes separated by gene deserts. In nuclei, this region forms multiple, nonrandom higher order structures. These structures are based on the gene distribution pattern in primary sequence and are marked by preferential associations among multiple gene clusters. Associating gene clusters represent expressed chromatin, but their aggregation is not simply dependent on ongoing transcription. In chromosomes with aggregated gene clusters, gene deserts preferentially align with the nuclear periphery, providing evidence for chromosomal region architecture by specific associations with functional nuclear domains. Together, these data suggest dynamic, probabilistic 3D folding states for a contiguous megabase-scale chromosomal region, supporting the diverse activities of multiple genes and their conserved primary sequence organization.
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