期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 27, 页码 10456-10460出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0603045103
关键词
adeno-associated virus; lordosis; RNA; interference viral vector
资金
- NIMH NIH HHS [MH62147, R01 MH062147, R03 MH067775, MH67775] Funding Source: Medline
- NINDS NIH HHS [K08 NS044978, NS044978] Funding Source: Medline
Estrogen receptor alpha (ER alpha) plays a major role in the regulation of neuroendocrine functions and behaviors by estrogens. Although the generation of ER alpha knockout mice advanced our knowledge of ER alpha functions, gene deletion using this method is global and potentially confounded by developmental consequences. To achieve a site-specific knockdown of ER alpha in the normally developed adult brain, we have generated an adeno-associated virus vector expressing a small hairpin RNA targeting ER alpha. After bilateral injection of this vector into the hypothalamic ventromedial nucleus in ovariectomized female mice, expression levels of ER alpha as well as the estrogen-inducible progesterone receptor were profoundly reduced despite the continued presence of this receptor elsewhere in the brain. Functionally, silencing of ER alpha in the ventromedial nucleus abolished female proceptive and receptive sexual behaviors while enhancing rejection behavior. These results provide evidence that adeno-associated virus-mediated long-term knockdown of genes can be used to delineate their effects on complex behaviors in discrete brain regions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据