期刊
JOURNAL OF NEUROSCIENCE
卷 26, 期 27, 页码 7230-7233出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1696-06.2006
关键词
chemosensitivity; purinergic signaling; central respiratory control; ventral medullary surface; pH signaling; brain slice
资金
- NHLBI NIH HHS [R01 HL074011, F32 HL80890, HL74011] Funding Source: Medline
- NINDS NIH HHS [R01 NS033583, NS33583] Funding Source: Medline
The cellular mechanism(s) by which the brain senses changes in pH to regulate breathing (i.e., central chemoreception) have remained incompletely understood, in large part because the central respiratory chemoreceptors have themselves eluded detection. Here, we recorded from a newly identified population of central chemoreceptors located in the retrotrapezoid nucleus (RTN) on the ventral surface of the brainstem to test a recently proposed role for purinergic P2 receptor signaling in central respiratory chemoreception (Gourine et al., 2005). Using loose-patch current-clamp recordings in brainstem slices from rat pups (postnatal day 7-12), we indeed show purinergic modulation of pH-sensitive RTN neurons: activation of P2X receptors indirectly inhibited RTN firing by increasing inhibitory input, whereas P2Y receptor stimulation caused direct excitation of RTN chemoreceptors. However, after blocking P2 receptors with the broad-spectrum antagonists PPADS (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate) or RB2 (reactive blue 2), the pH sensitivity of RTN neurons remained intact. Therefore, we conclude that purinergic signaling can modulate RTN neuron activity but does not mediate the pH sensing intrinsic to these central respiratory chemoreceptors.
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