期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1120, 期 1-2, 页码 165-172出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.01.038
关键词
monolith; proteomics; mass spectrometry; HPLC; biomarker; high-throughput peptide screening
Proteomic studies have stimulated the development of novel stationary phases in miniaturised chromatographic columns that permit high linear flow velocities and exhibit high resolving power. In this work, a 50-mu m reversed-phase silica-based monolith was chromatographically characterised for its use in proteomics applications using a nanoLC-MS set-up. It showed high efficiency for the separation of tryptic peptides under isocratic elution conditions (HETPmin = 5-10 mu m at 2.4 mm/s). Flow rates up to 1.95 mu L/min (18.4 mm/s) and gradient slopes up to an unusually fast 9% could be used. This resulted in rapid separations of peptide mixtures, with peak widths at half height of between 5 and 10 s. The 50-mu m monolithic column was used to analyse depleted serum from a cervical cancer patient at a throughput of one sample per 30 min. (c) 2006 Elsevier B.V. All rights reserved.
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