期刊
CELL
卷 126, 期 1, 页码 191-203出版社
CELL PRESS
DOI: 10.1016/j.cell.2006.04.045
关键词
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资金
- NIGMS NIH HHS [R01 GM44530, P01 GM65533, P01 GM065533] Funding Source: Medline
In the yeast Saccharomyces cerevisiae, the G protein beta gamma subunits are essential for pheromone signaling. The G alpha subunit Gpa1 can also promote signaling, but the effectors in this pathway are not well characterized. To identify candidate Gpa1 effectors, we expressed the constitutively active Gpa1(Q323L) mutant in each of nearly 5000 gene-deletion strains and measured mating-specific responses. Our analysis reveals a requirement for both the catalytic (Vps34) and regulatory (Vps15) subunits of the sole phosphaticlylinositol 3-kinase in yeast. We demonstrate that Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidlylinositol 3-phosphate. Notably, Vps15 binds to GDP-bound Gpa1 and is predicted to have a seven-WID repeat structure similar to that of known G protein beta subunits. These findings reveal two new components of the pheromone signaling pathway. More remarkably, these proteins appear to comprise a preformed effector-G beta subunit assembly and function at the endosome rather than at the plasma membrane.
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