Electroporation of mammalian cells in a microfluidic channel with geometric variation

Electroporation has been widely used to load impermeant exogenous molecules into cells. Rapid electrical lysis based on electroporation has also been applied to analyze intracellular materials at single-cell level. There has been increasing demand to implement electroporation in a microfluidic format as a basic tool for applications ranging from screening of drugs and genes to studies of intracellular dynamics. In this report, we have developed a simple technique to electroporate mammalian cells with high throughput on a microfluidic platform. In our design, electroporation only happened in a defined section of a microfluidic channel due to the local field amplification by geometric variation. The time of exposure of the cells to this high field was determined by the velocity of the cells and the length of the section. The change in the cell morphology during electroporation was observed in real time. We determined that electroporation of Chinese hamster ovary cells occurred when the local field strength was increased to similar to 400 V/cm. The internalization of membrane-impermeant molecules ( SYTOX green) with cell viability preserved was also carried out to demonstrate transient electropermeabilization. The influence of the operational parameters of the device on cell viability was determined. A large percentage of cells remained viable after electroporation when the parameters were tuned. We also studied rapid cell lysis when the field intensity was in the range of 600-1200 V/cm. The rupture of cell membrane happened within 30 ms when the field strength was 1200 V/cm. Given the simplicity, high throughput, and high compatibility with other devices, this microfluidic electroporation technique may increase the application of microfluidic systems in screening of drugs and biomolecules and chemical cytometry.