期刊
TALANTA
卷 69, 期 5, 页码 1162-1165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2005.12.041
关键词
metallothionein; bismuth film electrode; differential pulse anodic stripping voltammetry
As a representation of metalloproteins, metallothionein (MT), which plays important biological and environmental roles such as in the metabolism and detoxification of some metals, was detected at bismuth film electrode (BiFE) by differential pulse anodic stripping voltammetry (DPASV). In pH 2-5.5, two well-defined anodic peaks were produced and attributed to the Zn2+ and Cd2+ inherent to MT The calibration plot of DPASV peak currents for Cd2+ inherent to MT versus MT concentrations showed a good linearity with a detection limit of 3.86 x 10(-8) mol/L for MT. As a non-toxic excellent electrode material, BiFE shows good performance for detecting MT, and is expected to find further applications in the studies of many other metalloproteins. (c) 2005 Elsevier B.V. All rights reserved.
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