期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 30, 页码 11411-11416出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0601631103
关键词
chloride channels; gating; kidney; ClC family; accessory subunit
Barttin is an accessory subunit of a subgroup of CIC-type chloride channels expressed in renal and inner ear epithelia. In this study, we examined the effects of barttin on two CIC-K channel isoforms, rat CIC-K1 and human CIC-Kb, using heterologous expression, patch clamping, confocal imaging, and flow cytometry. In the absence of barttin, only a small percentage of rCIC-K1 and hCIC-Kb channels are inserted into the plasma membrane. Coexpression of barttin enhances surface membrane insertion and furthermore modifies permeation and gating of CIC-K channels. hCIC-Kb channels are nonfunctional without barttin and require the coexpressed accessory subunit to become anion conducting. In contrast, rCIC-K1 channels are active without barttin, but at the cost of reduced unitary conductance as well as altered voltage dependence of activation. We mapped the separate functions of barttin to structural domains by a deletion analysis. Whereas the transmembrane core is necessary and sufficient to promote CIC-K channel exit from the endoplasmic reticulum, a short cytoplasmic segment following the second transmembrane helix modifies the unitary conductance. The entire cytoplasmic carboxyl terminus affects the open probability of CIC-K channels. The multiple functions of barttin might be necessary for a tight adjustment of epithelial Cl-conductances to ensure a precise regulation of body salt content and endocochlear potential.
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