期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 30, 页码 11142-11147出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0604075103
关键词
membrane proteins; membrane proteomics; yeast
The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximate to 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.
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