期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 68, 期 1, 页码 1-21出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbbm.2006.02.011
关键词
biomagnetic separation; antibody binding; magnetophoresis; CD45; immunomagnetic cell separation; cell sorting
资金
- NCI NIH HHS [R01 CA97391, R01 CA62349] Funding Source: Medline
Cell separation is important in medical and biological research and plays an increasingly important role in clinical therapy and diagnostics. such as rare cancer cell detection in blood. The immunomagnetic labeling of cells with antibodies conjugated to magnetic nanospheres gives rise to a proportional relationship between the number of magnetic nanospheres attached to the cell and the cell surface marker number. This enables the potential fractionation of cell populations by magnetophoretic mobility (MM). We exploit this feature with our apparatus, the Dipole Magnet Flow Fractionator (DMFF), which consists of an isodynamic magnetic field. an orthogonally-oriented thin ribbon of cell suspension in continuous sheath flow, and ten outlet flows. From a sample containing a 1:1 mixture of immunomagnetically labeled (label+) and unlabeled (label-) cells, we achieved an increase in enrichment of the label+. cell fraction with increasing outlet numbers in the direction of the magnetic field gradient (up to 10-fold). The total recovery of the ten Outlet fractions was 90.0 +/- 7.7%. The mean MM of label+ cells increased with increasing outlet number by up to a factor of 2.3. The postulated proportionalin between the number of attached magnetic beads and the number of cell surface markers was validated by comparison of MM measured by cell tracking velocimetry (CTV) with cell florescence intensity measured by flow cytometry. (c) 2006 Elsevier B.V. All rights reserved.
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