期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 24, 期 8, 页码 1782-1790出版社
JOHN WILEY & SONS INC
DOI: 10.1002/jor.20211
关键词
cyclic load; oxidized low density lipoprotein (ox-LDL); lectin-like oxidized LDL receptor-1 (LOX-1); chondrocytes; cell viability
类别
Mechanical stimulation is known to be an essential factor in the regulation of cartilage metabolism. We tested the hypothesis that expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) can be modulated by cyclic tensile stretch load in chondrocytes. Cyclic loading of repeated stretch stress at 10 cycles per minute with 10 kPa of stress for 6 h induced expression of LOX-1 to 2.6 times control in cultured bovine articular chondrocytes, equivalent to the addition of 10 mu g/mL oxidized low density lipoprotein (ox-LDL) (2.4 times control). Application of the cyclic load to the chondrocytes along with 10 mu g/mL ox-LDL resulted in synergistically increased LOX-1 expression to 6.3 times control. Individual application of cyclic loading and 10 mu g/mL ox-LDL significantly suppressed chondrocytes viability (84.6% 3.4% and 80.9% 3.2% of control at 24 h, respectively; n = 3; p < 0.05) and proteoglycan synthesis [81.0% 7.1% and 85.7% 5.2% of control at 24 h, respectively; p < 0.05 when compared with 94.6% 4.6% for native-LDL (n = 3)]. Cyclic loading and 10 mu g/mL ox-LDL synergistically affected cell viability and proteoglycan synthesis, which were significantly suppressed to 45.6% 4.9% and 48.7% 6.7% of control at 24 h, respectively (n 3; p < 0.01 when compared with individual application of cyclic loading or 10 mu g/mL ox-LDL). In this study, we demonstrated synergistic effects of cyclic tensile stretch load and ox-LDL on cell viability and proteoglycan synthesis in chondrocytes, which may be mediated through enhanced expression of LOX-1 and which has important implications in the progression of cartilage degeneration in osteoarthritis. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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