Shimia marina gen. nov., sp nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm

A rod-shaped marine bacterium, CL-TA03(T), isolated from a biofilm in a coastal fish farm in Tongyeong, Korea, was characterized for physiological and biochemical features, fatty acid profile and phylogenetic position based on 16S rRNA gene sequences. Analysis of the 1 6S rRNA gene sequence revealed a clear affiliation with the family Rhodobacteraceae. Phylogenetic analysis of the 1 6S rRNA gene sequence showed that the closest relatives of CL-TA03(T) were Thalassobius gelatinovorus and Thalassobius mediterraneus (95-6% similarity). The sequence similarities between CL-TA03(T) and other type species of the Roseobacter lineage ranged from 92-4 to 95-4%. Strain CL-TA03(T) is motile and grows on marine agar as colourless or beige colonies. The strain is able to grow optimally in the range of 3-5% sea salts. It grows within a temperature range of 15-35 degrees C and at pH 6-10. The fatty acids are dominated by 18: 1 omega 7c (64-1%) and 11-methyl 18: 1 omega 7c (10-6%). The DNA G+C content is 57-2 mol%. According to physiological data, fatty acid composition and phylogenetic analysis of the 1 6S rRNA gene sequence, CL-TA03(T) is considered to represent a new genus in the family Rhodobacteraceae and the name Shimia marina gen. nov., sp. nov. is proposed. The type strain of Shimia marina is CL-TA03(T) (= KCCM 42117(T)=JCM 13038(T)).